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anti sdhc  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology anti sdhc
    Anti Sdhc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sc+515102/SDHC+Antibody/pmc12678531-36-0-2
    Average 93 stars, based on 14 article reviews
    anti sdhc - by Bioz Stars, 2026-09
    93/100 stars

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    Article Title: Beta cell dysfunction occurs independently of insulitis in type 1 diabetes pathogenesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Human CD39L3/ENTPD3 Antibody R and D Systems Cat# AF4400 RRID: AB_11127454 Fluo-4, AM ThermoFisher Scientific Cat# F14201 SYTOX Blue Fisher Scientific Cat# 501137613 Calbryte 520 AM AAT Bioquest Cat# 20650 Alexa Fluor 647 anti-human CD3 Antibody Biolegend Cat# 300416 Clone UCHT1 RRID: AB_389332 Nuclear Yellow Abcam Cat# ab138903 ATPIF1 Monoclonal Antibody Invitrogen Cat# MA5-31642 Clone F3B8 RRID: AB_2787266 ATP Synthase Beta Monoclonal Antibody Invitrogen Cat# A-21351 Clone 3D5 RRID: AB_221512 Polyclonal Rabbit Anti-Human CD3 Antibody Agilent Dako Cat# A0452 RRID: AB_2335677 BD Pharmingen TM Alexa Fluor® 647 Mouse Anti-Insulin BD Biosciences Cat# 565689 Clone T56-706 RRID: AB_2739331 Kir6.2 (KCNJ11) Polyclonal Antibody Invitrogen Cat# PA5-99440 RRID: AB_2818373 SDHC Antibody Santa Cruz Biotechnology Cat# sc-515102 Clone C-2 RRID: AB_2890985 PCB Antibody Santa Cruz Biotechnology Cat# sc-271493 Clone H-2 RRID: AB_10649369 GLUT1 Recombinant Rabbit Monoclonal Antibody Invitrogen Cat# MA5-31960 Clone SA0377 RRID: AB_2809254 GCK Antibody Santa Cruz Biotechnology Cat# sc-17819 Clone G-6 RRID: AB_627722 CACNA1D Polyclonal Antibody Proteintech Cat# 22276-1-AP RRID: AB_2879057 Polyclonal Rabbit anti-Human EIF2AK3/PERK Antibody LS Bio Cat# LS-C353177 RRID: AB_3678741 NDUFS1 Polyclonal Antibody Proteintech Cat# 12444-1-AP RRID: AB_2282657 COX11 Polyclonal Antibody Invitrogen Cat# PA5-104519 RRID: AB_2853820 IDH3A Antibody Santa Cruz Biotechnology Cat# sc-398021 Clone A-10 RRID: AB_2940886 GAPDH Antibody Santa Cruz Biotechnology Cat# sc-47724 Clone 0411 RRID: AB_627678 OGDH Monoclonal Antibody Proteintech Cat# 66285-1-Ig Clone 1H7A2 RRID: AB_2881668 UQCRQ Polyclonal Antibody Invitrogen Cat# PA5-106446 RRID: AB_2854117 CD45 Rabbit Monoclonal Antibody Cell Signaling Technology Cat# 13917T Clone D9M8I RRID: AB_2750898 (Continued on next page) 16 Cell Reports 44, 116174, September 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Borg Decloaker Biocare Medical Cat# BD1000G1 Opal Antibody Diluent/Block Akoya Biosciences Cat# ARD1001EA Deoxyribonuclease I, Recombinant, AF Worthington Biochemical Corporation Cat# 9003-98-9 Critical commercial assays Insulin ELISA Mercodia Cat# 10-1113-10 Ultrasensitive Insulin ELISA Mercodia Cat# 10-1132-01 PicoPure RNA Isolation Kit ThermoFisher Scientific Cat# KIT0204 Affymetrix Human Gene 2.0 ST expression array ThermoFisher Scientific Cat# 902112 Deposited data Ca 2+ Recording Analysis Code This paper https://doi.org/10.5281/zenodo.14605547 RNA Expression Dataset This paper GEO accession number: GSE284772 260 Islets Expression Matrix This paper https://doi.org/10.5281/zenodo.14537115 RNA Expression Analysis R Code This paper https://doi.org/10.5281/zenodo.14537115 Perifusion Analysis R Code This paper https://doi.org/10.5281/zenodo.14605547 Slice Ca 2+ Videos This paper https://doi.org/10.5281/zenodo.14605547 Software and algorithms ImageJ Schneider et al. 72 https://imagej.net/ij/ FIJI Schindelin et al. 73 https://imagej.net/software/fiji/ Trackmate Ershov et al. 74 https://imagej.net/plugins/trackmate/ StackReg Thevenaz, P., Ruttimann, U. E. and Unser, M 75 https://bigwww.epfl.ch/thevenaz/stackreg/ RStudio v2024.04.234 https://posit.co/products/open-source/rstudio/ nlme Package J Pinheiro 76 https://cran.r-project.org/web/ packages/nlme/index.htm lsmeans Package Lenth, R. V. 77 https://www.jstatsoft.org/article/view/v069i01 Cell Profiler Stirling et al. 534 https://cellprofiler.org ComplexHeatmap Package v2.20.0 Gu,Z, 78 Gu, Z., Eils, R. & Schlesner, M 79 https://github.com/jokergoo/ComplexHeatmap vsn Package Huber et al. 80 https://bioconductor.org/packages/ release/bioc/html/vsn.html M3C package v1.26.0 John et al. 81 https://www.bioconductor.org/ packages/3.7/bioc/html/M3C.html Prism software version 10.3.1.



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    Figure 3. Male hepatic gene expression and protein abundance of four proteins in oxidative phospho- rylation complexes in the control (C) and maternal obesity (MO) groups. Gene expression level (Log2 RPKM) of (A) ndufa10; (B) <t>sdhc;</t> <t>(C)</t> <t>cox5a;</t> (D) atp5f1; the immunostained area (%) of (E) Ndufa10; (F) Sdhc; (G) Cox5a; (H) Atp5f1; and (I) representative IHC micrograph (40×). Data for RNA-seq, mean Log2 RPKM ± SEM. Protein values are mean ± SEM. p < 0.05 for data not sharing a lowe case letter between groups. N = 5–6 rats/group/litter. PND = Postnatal days. Scale bar: 50 µm.
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    Prognostic significance of SDHs. (A) Venn diagram of DEGs in CHOL, COAD, LIHC, READ, and STAD. (B) Venn diagram of DEGs, MMGs, and HIF-1α-related genes. (C) The network for 8 DEGs intersected. (D) Heatmap of SDHA, SDHB, <t>SDHC,</t> and SDHD between 41 normal tissues and 453 COAD patients. (E-H) Kaplan-Meier overall survival of SDHA, SDHB, SDHC, and SDHD in COAD.
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    Prognostic significance of SDHs. (A) Venn diagram of DEGs in CHOL, COAD, LIHC, READ, and STAD. (B) Venn diagram of DEGs, MMGs, and HIF-1α-related genes. (C) The network for 8 DEGs intersected. (D) Heatmap of SDHA, SDHB, <t>SDHC,</t> and SDHD between 41 normal tissues and 453 COAD patients. (E-H) Kaplan-Meier overall survival of SDHA, SDHB, SDHC, and SDHD in COAD.
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    Figure 3. Male hepatic gene expression and protein abundance of four proteins in oxidative phospho- rylation complexes in the control (C) and maternal obesity (MO) groups. Gene expression level (Log2 RPKM) of (A) ndufa10; (B) sdhc; (C) cox5a; (D) atp5f1; the immunostained area (%) of (E) Ndufa10; (F) Sdhc; (G) Cox5a; (H) Atp5f1; and (I) representative IHC micrograph (40×). Data for RNA-seq, mean Log2 RPKM ± SEM. Protein values are mean ± SEM. p < 0.05 for data not sharing a lowe case letter between groups. N = 5–6 rats/group/litter. PND = Postnatal days. Scale bar: 50 µm.

    Journal: Biology

    Article Title: Maternal Obesity Programs the Premature Aging of Rat Offspring Liver Mitochondrial Electron Transport Chain Genes in a Sex-Dependent Manner.

    doi: 10.3390/biology12091166

    Figure Lengend Snippet: Figure 3. Male hepatic gene expression and protein abundance of four proteins in oxidative phospho- rylation complexes in the control (C) and maternal obesity (MO) groups. Gene expression level (Log2 RPKM) of (A) ndufa10; (B) sdhc; (C) cox5a; (D) atp5f1; the immunostained area (%) of (E) Ndufa10; (F) Sdhc; (G) Cox5a; (H) Atp5f1; and (I) representative IHC micrograph (40×). Data for RNA-seq, mean Log2 RPKM ± SEM. Protein values are mean ± SEM. p < 0.05 for data not sharing a lowe case letter between groups. N = 5–6 rats/group/litter. PND = Postnatal days. Scale bar: 50 µm.

    Article Snippet: The following primary antibodies were used for IHC analysis: anti Atp5f1, 1:500 (goat polyclonal SC-162552, Santa Cruz Biotechnology, Dallas, TX, USA); anti Ndufa10, 1:500 (mouse monoclonal SC-376357, Santa Cruz); anti Cox5a, 1:100 (mouse monoclonal SC-376907, Santa Cruz Biotechnology); anti Sdhc, 1:1000 (rabbit polyclonal SC-67256, Santa Cruz Biotechnology); anti Sirt-2, 1:1000 (mouse monoclonal SC-28292, Santa Cruz Biotechnology); anti Sirt-3, 1:300 (rabbit Biology 2023, 12, 1166 5 of 22 polyclonal SC-99143, Santa Cruz Biotechnology); anti Sod-1, 1:1000 (rabbit polyclonal SC11407, Santa Cruz Biotechnology); Catalase, 1:600 (rabbit polyclonal SC-50508, Santa Cruz Biotechnology).

    Techniques: Gene Expression, Quantitative Proteomics, Control, RNA Sequencing

    Figure 4. Female hepatic changes in gene expression and abundance of proteins in four oxidative phosphorylation complexes in the control (C) and maternal obesity (MO) groups. Gene expres- sion (Log2 RPKM) of (A) ndufa10; (B) sdhc; (C) cox5a; (D) atp5f1; the immunostained area (%) of (E) Ndufa10; (F) Sdhc; (G) Cox5a; (H) Atp5f1; and (I) representative IHC micrograph (40×). Data for RNA-seq, mean Log2 RPKM ± SEM. Protein values are mean ± SEM. p < 0.05 for data not sharing a lower case letter between groups. n = 5–6 rats/group/litter. PND = Postnatal days. Scale bar: 50 µm.

    Journal: Biology

    Article Title: Maternal Obesity Programs the Premature Aging of Rat Offspring Liver Mitochondrial Electron Transport Chain Genes in a Sex-Dependent Manner.

    doi: 10.3390/biology12091166

    Figure Lengend Snippet: Figure 4. Female hepatic changes in gene expression and abundance of proteins in four oxidative phosphorylation complexes in the control (C) and maternal obesity (MO) groups. Gene expres- sion (Log2 RPKM) of (A) ndufa10; (B) sdhc; (C) cox5a; (D) atp5f1; the immunostained area (%) of (E) Ndufa10; (F) Sdhc; (G) Cox5a; (H) Atp5f1; and (I) representative IHC micrograph (40×). Data for RNA-seq, mean Log2 RPKM ± SEM. Protein values are mean ± SEM. p < 0.05 for data not sharing a lower case letter between groups. n = 5–6 rats/group/litter. PND = Postnatal days. Scale bar: 50 µm.

    Article Snippet: The following primary antibodies were used for IHC analysis: anti Atp5f1, 1:500 (goat polyclonal SC-162552, Santa Cruz Biotechnology, Dallas, TX, USA); anti Ndufa10, 1:500 (mouse monoclonal SC-376357, Santa Cruz); anti Cox5a, 1:100 (mouse monoclonal SC-376907, Santa Cruz Biotechnology); anti Sdhc, 1:1000 (rabbit polyclonal SC-67256, Santa Cruz Biotechnology); anti Sirt-2, 1:1000 (mouse monoclonal SC-28292, Santa Cruz Biotechnology); anti Sirt-3, 1:300 (rabbit Biology 2023, 12, 1166 5 of 22 polyclonal SC-99143, Santa Cruz Biotechnology); anti Sod-1, 1:1000 (rabbit polyclonal SC11407, Santa Cruz Biotechnology); Catalase, 1:600 (rabbit polyclonal SC-50508, Santa Cruz Biotechnology).

    Techniques: Gene Expression, Phospho-proteomics, Control, RNA Sequencing

    Prognostic significance of SDHs. (A) Venn diagram of DEGs in CHOL, COAD, LIHC, READ, and STAD. (B) Venn diagram of DEGs, MMGs, and HIF-1α-related genes. (C) The network for 8 DEGs intersected. (D) Heatmap of SDHA, SDHB, SDHC, and SDHD between 41 normal tissues and 453 COAD patients. (E-H) Kaplan-Meier overall survival of SDHA, SDHB, SDHC, and SDHD in COAD.

    Journal: Frontiers in Immunology

    Article Title: Comprehensive analysis of the prognosis, tumor microenvironment, and immunotherapy response of SDHs in colon adenocarcinoma

    doi: 10.3389/fimmu.2023.1093974

    Figure Lengend Snippet: Prognostic significance of SDHs. (A) Venn diagram of DEGs in CHOL, COAD, LIHC, READ, and STAD. (B) Venn diagram of DEGs, MMGs, and HIF-1α-related genes. (C) The network for 8 DEGs intersected. (D) Heatmap of SDHA, SDHB, SDHC, and SDHD between 41 normal tissues and 453 COAD patients. (E-H) Kaplan-Meier overall survival of SDHA, SDHB, SDHC, and SDHD in COAD.

    Article Snippet: Then, the tissue sections were incubated overnight with rabbit monoclonal anti-SDHA antibody (1:100 dilution, Proteintech), rabbit monoclonal anti-SDHB antibody (1:100 dilution, Santa cruz), rabbit monoclonal anti-SDHC antibody (1:100 dilution, Proteintech), and rabbit monoclonal anti-SDHD antibody (1:100 dilution, Affbiotech) at 4°C, respectively.

    Techniques:

    SDH factor expression and the clinical characteristics of patients with COAD. (A-E) SDHA, SDHB, SDHC, and SDHD are concerned with clinical characteristics involving T stage, N stage, M stage, age, and lymphatic invasion. (F) A nomogram to predict the overall survival rate of COAD patients. (G-J) Time-dependent ROC analysis and Calibration curve for the overall survival nomogram model in the discovery group. A dashed diagonal line represents the ideal nomogram. *p < 0.05; **p < 0.01 and ***p < 0.001; ns, not significant.

    Journal: Frontiers in Immunology

    Article Title: Comprehensive analysis of the prognosis, tumor microenvironment, and immunotherapy response of SDHs in colon adenocarcinoma

    doi: 10.3389/fimmu.2023.1093974

    Figure Lengend Snippet: SDH factor expression and the clinical characteristics of patients with COAD. (A-E) SDHA, SDHB, SDHC, and SDHD are concerned with clinical characteristics involving T stage, N stage, M stage, age, and lymphatic invasion. (F) A nomogram to predict the overall survival rate of COAD patients. (G-J) Time-dependent ROC analysis and Calibration curve for the overall survival nomogram model in the discovery group. A dashed diagonal line represents the ideal nomogram. *p < 0.05; **p < 0.01 and ***p < 0.001; ns, not significant.

    Article Snippet: Then, the tissue sections were incubated overnight with rabbit monoclonal anti-SDHA antibody (1:100 dilution, Proteintech), rabbit monoclonal anti-SDHB antibody (1:100 dilution, Santa cruz), rabbit monoclonal anti-SDHC antibody (1:100 dilution, Proteintech), and rabbit monoclonal anti-SDHD antibody (1:100 dilution, Affbiotech) at 4°C, respectively.

    Techniques: Expressing

    Univariate and multivariate Cox regression analysis of SDHs for COAD with clinical characteristics in TCGA cohort.

    Journal: Frontiers in Immunology

    Article Title: Comprehensive analysis of the prognosis, tumor microenvironment, and immunotherapy response of SDHs in colon adenocarcinoma

    doi: 10.3389/fimmu.2023.1093974

    Figure Lengend Snippet: Univariate and multivariate Cox regression analysis of SDHs for COAD with clinical characteristics in TCGA cohort.

    Article Snippet: Then, the tissue sections were incubated overnight with rabbit monoclonal anti-SDHA antibody (1:100 dilution, Proteintech), rabbit monoclonal anti-SDHB antibody (1:100 dilution, Santa cruz), rabbit monoclonal anti-SDHC antibody (1:100 dilution, Proteintech), and rabbit monoclonal anti-SDHD antibody (1:100 dilution, Affbiotech) at 4°C, respectively.

    Techniques: